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KMID : 0363620190400020051
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Journal of Korean Oriental Medicine
2019 Volume.40 No. 2 p.51 ~ p.62
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Effects of 『Geum-Gwe-Yo-Ryak(金?要略)』 Prescription for Chest Pain Including Kwaruhaebaekbanha-tang and Kwaruhaebaekpaekju-tang on Macrophage Polarization
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Son Chang-Hyeon
Lee Sang-Min
Yu Ga-Ram
Lee Seung-Jun
Lim Dong-Woo
Kim Hyuck
Park Won-Hwan
Kim Jae-Eun
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Abstract
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Objectives: This study was designed to evaluate the macrophages polarization of traditional Korean medicine on cardiac pain about Geum-Gwe-Yo-Ryak's two prescriptions including Kwaruhaebaekbanha-tang (KHB) and Kwaruhaebaekpaekju-tang (KHP).
Materials and methods: Flow cytometry analysis was used to measure the changes in the ratio of M1 type and M2 type macrophages. Protein expression of nuclear factor-like 2 (Nrf2), heme oxygenase-1 (HO-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were measured by Western Blot, and ABCA1 and SR-B1 were detected by real time PCR (RT-PCR). Intracellular lipid accumulation was measured by Oil Red O staining (ORO staining).
Results: KHB and KHP increase anti-oxidative activity related protein levels including Nrf2 and HO-1. Furthermore, KHB and KHP inhibit lipid accumulation on intracellular levels through induction of ATP binding receptor cassette subfamily A member 1 (ABCA1) and scavenging receptor class B member 1 (SR-B1), respectively. Finally, KHB and KHP also blocked pro-inflammatory mediators including tumor necrosis factor-alpha (TNFα) and interleukin-6 (IL-6), iNOS and COX-2 expression.
Conclusion: This study suggests that KHB and KHP potently regulate the M1/M2 macrophage polarization.
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KEYWORD
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Geum-Gwe-Yo-Ryak, Kwaruhaebaekbanha-tang, Kwaruhaebaekpaekju-tang, Macrophage polarization
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